Bacterial Glycocalyx Integrity Impacts Tolerance of Myxococcus xanthus to Antibiotics and Oxidative-Stress Agents

Biomolecules

Salim T. Islam¹  Nicholas Y. Bradette¹  Razieh Bitazar¹  Fares Saïdi¹ 

[1] Centre Armand-Frappier Santé Biotechnologie, Institut National de la Recherche Scientifique (INRS), Université du Québec, Institut Pasteur International Network, Laval, QC H7V 1B7, Canada;

关键词: biofilms;    extracellular matrix;    antibiotic resistance;    antibiotic tolerance;    reactive oxygen species (ROS);    Wzx/Wzy-dependent pathway;   

DOI  :  10.3390/biom12040571

来源: DOAJ

【 摘 要 】

The presence of an exopolysaccharide (EPS) layer surrounding bacterial cells, termed a “glycocalyx”, confers protection against toxic molecules. However, the effect of glycocalyx integrity on the tolerance to such agents is poorly understood. Using a modified disc-diffusion assay, we tested the susceptibility to a panel of antibiotics and oxidative stress-inducing compounds of various mutant strains of the social predatory Gram-negative soil bacterium Myxococcus xanthus; the selected mutants were those that manifest different physical states of their respective EPS glycocalyces. While the overall presence of an EPS layer was indeed beneficial for tolerance, the integrity of this layer was also found to affect the susceptibility of the bacterium to killing; however, this finding was not universal, and instead was dependent on the specific compound tested. Thus, the integrity of the cell-surface EPS glycocalyx plays an important role in the tolerance of M. xanthus to harmful compounds.

【 授权许可】

Unknown