Sequencing of third exon of IGF1 gene in sheep

مجله بیوتکنولوژی کشاورزی

Mohammad Reza Bahreini Behzadi¹  Saeideh Sajjadi²  Majid Fardaei³ 

[1] استادیار گروه علوم دامی، دانشکده کشاورزی، دانشگاه یاسوج;دانشجوی کارشناسی ارشد اصلاح نژاد دام، دانشکده کشاورزی، دانشگاه یاسوج;دانشیار گروه ژنتیک پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکی شیراز;

关键词: sequencing;    polymorphism;    igf1 gene;    protein structure;    sheep;   

DOI  :  10.22103/jab.2016.1392

来源: DOAJ

【 摘 要 】

Insulin-like growth factor is a single-chain polypeptide. The Molecular weight of it is 7.5 KDa. This factor is insulin like activity and the most important growth factor in the body and plays important role in the proliferation, differentiation and cells growth. IGF1 gene located on chromosome 3 in sheep. This gene is more proposed as a candida gene for growth and meat quality traits in animal genetic improvement programs. The aim of this research is to find Polymorphisms in the third exon. In this research blood samples of breeds of 25 sheep include Mehraban, Karakul, Ghezel, Lak Ghashghaei and Bahmaei were collected with EDTA tube. The DNA was extracted from blood samples and the third exon region was amplified with specific primers using PCR and sequencing. The results showed, there is 2 SNP in intron 2 and 3. Protein structure of IGF1 gene is investigated by using Swiss-pdb viewer software. Protein structure analysis showed that this protein is highly conserved of evolution. This protein has a structure consisting of an alpha helix with irregular structure.

【 授权许可】

Unknown