Molecular Markers for Identifying Resistance Genes in Brassica napus

Agronomy

Elizabeth Sheedy¹  Angela P. Van de Wouw²  Candace E. Elliott²  Nur Shuhadah Mohd Saad³  Jacqueline Batley³  Yueqi Zhang³  Hua Yang³ 

[1] Marcroft Grains Pathology, Grains Innovation Park, Horsham, VIC 3400, Australia;School of BioSciences, University of Melbourne, Parkville, Melbourne, VIC 3010, Australia;School of Biological Sciences, The University of Western Australia, Crawley, WA 6009, Australia;

关键词: Kompetitive Allele-Specific PCR (KASP);    blackleg;    Leptosphaeria maculans;    canola;    molecular breeding;   

DOI  :  10.3390/agronomy12050985

来源: DOAJ

【 摘 要 】

Blackleg disease, caused by the fungal pathogen Leptosphaeria maculans, is the most devastating disease of canola (Brassica napus, oilseed rape) worldwide. Breeding for genetic resistance is the most widely used tool for controlling this disease and minimizing the impact on yield. To date, five resistance genes (Rlm2, LepR3, Rlm4, Rlm7, Rlm9) have been cloned from B. napus, representing alleles of two different gene loci, Rlm2-LepR3 and Rlm4-7-9. We report on the development and validation of Kompetitive Allele-Specific PCR (KASP) markers that can discriminate between the resistant and susceptible alleles of each resistance gene. These markers will provide valuable tools for both researchers and industry through the ability to characterize resistance genes without phenotyping.

【 授权许可】

Unknown