Chromosomal assembly of the Antarctic toothfish (Dissostichus mawsoni) genome using third-generation DNA sequencing and Hi-C technology

Zoological Research

Eunkyung Choi¹  Seung Jae Lee¹  Jinmu Kim¹  Hyun Park¹  Euna Jo¹  Hyun-Woo Kim²  Jeong-Hoon Kim³  Seok-Gwan Choi⁴  Sangdeok Chung⁴ 

[1] College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea;Department of Marine Biology, Pukyong National University, Busan 48513, Republic of Korea;Division of Polar Life Science, Korea Polar Research Institute, Incheon 21990, Republic of Korea;National Institute of Fisheries Science (NIFS), Busan 46083, Republic of Korea;

关键词: antarctic toothfish;    dissostichus mawsoni;    genome assembly;    pacific biosciences;    hi-c;   

DOI  :  10.24272/j.issn.2095-8137.2020.264

来源: DOAJ

【 摘 要 】

The Antarctic toothfish, Dissostichus mawsoni, belongs to the Nototheniidae family and is distributed in sub-zero temperatures below S60° latitude in the Southern Ocean. Therefore, it is an attractive model species to study the stenothermal cold-adapted character state. In this study, we successfully generated highly contiguous genome sequences of D. mawsoni, which contained 1 062 scaffolds with a N50 length of 36.98 Mb and longest scaffold length of 46.82 Mb. Repetitive elements accounted for 40.87% of the genome. We also inferred 32 914 protein-coding genes using in silico gene prediction and transcriptome sequencing and detected splicing variants using Isoform-Sequencing (Iso-Seq), which will be invaluable resource for further exploration of the adaptation mechanisms of Antarctic toothfish. This new high-quality reference genome of D. mawsoni provides a fundamental resource for a deeper understanding of cold adaptation and conservation of species.

【 授权许可】

Unknown