Monitoring NAD(H) and NADP(H) dynamics during organismal development with genetically encoded fluorescent biosensors

Cell Regeneration

Yi Yang¹  Shuning Liu¹  Ting Li¹  Yejun Zou¹  Yuzheng Zhao¹  Zhuo Zhang¹ 

[1] Optogenetics & Synthetic Biology Interdisciplinary Research Center, State Key Laboratory of Bioreactor Engineering, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, East China University of Science and Technology;

关键词: Cell metabolism;    NAD(H) and NADP(H);    Genetically encoded fluorescent sensors;    Real-time monitoring;    Organismal development;   

DOI  :  10.1186/s13619-021-00105-4

来源: DOAJ

【 摘 要 】

Abstract Cell metabolism plays vital roles in organismal development, but it has been much less studied than transcriptional and epigenetic control of developmental programs. The difficulty might be largely attributed to the lack of in situ metabolite assays. Genetically encoded fluorescent sensors are powerful tools for noninvasive metabolic monitoring in living cells and in vivo by highly spatiotemporal visualization. Among all living organisms, the NAD(H) and NADP(H) pools are essential for maintaining redox homeostasis and for modulating cellular metabolism. Here, we introduce NAD(H) and NADP(H) biosensors, present example assays in developing organisms, and describe promising prospects for how sensors contribute to developmental biology research.

【 授权许可】

Unknown