Non-Coding, RNAPII-Dependent Transcription at the Promoters of rRNA Genes Regulates Their Chromatin State in S. cerevisiae

Non-Coding RNA

Christophe Dez¹  Sophie Queille¹  Emma Lesage¹  Marta Kwapisz¹  Olivier Gadal¹  Jorge Perez-Fernandez¹ 

[1] Laboratoire de Biologie Moléculaire, Cellulaire et du Développement (MCD), Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, 31062 Toulouse, France;

关键词: UPS;    RNA polymerase I (RNAPI);    RNA polymerase II (RNAPII);    ribosomal DNA (rDNA);    ribosomal RNA (rRNA);    long non-coding RNA (lncRNA);   

DOI  :  10.3390/ncrna7030041

来源: DOAJ

【 摘 要 】

Pervasive transcription is widespread in eukaryotes, generating large families of non-coding RNAs. Such pervasive transcription is a key player in the regulatory pathways controlling chromatin state and gene expression. Here, we describe long non-coding RNAs generated from the ribosomal RNA gene promoter called UPStream-initiating transcripts (UPS). In yeast, rDNA genes are organized in tandem repeats in at least two different chromatin states, either transcribed and largely depleted of nucleosomes (open) or assembled in regular arrays of nucleosomes (closed). The production of UPS transcripts by RNA Polymerase II from endogenous rDNA genes was initially documented in mutants defective for rRNA production by RNA polymerase I. We show here that UPS are produced in wild-type cells from closed rDNA genes but are hidden within the enormous production of rRNA. UPS levels are increased when rDNA chromatin states are modified at high temperatures or entering/leaving quiescence. We discuss their role in the regulation of rDNA chromatin states and rRNA production.

【 授权许可】

Unknown