Evaluation of the ex vivo Effects of Tamoxifen on Adipose-Derived Stem Cells: A Pilot Study

Frontiers in Cell and Developmental Biology

Andrea Gerardo Antonio Lania¹  Ilena Boemi³  Eleonora Vitali⁴  Nurçin Liman⁴  Luca Maione⁵  Marco Ettore Attilio Klinger⁶  Andrea Vittorio Emanuele Lisa⁶  Federico Barbera⁶  Valeriano Vinci⁶  Andrea Battistini⁶ 

[1] Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, Italy;Department of Medical Biotechnology and Translational Medicine BIOMETRA, Reconstructive and Aesthetic Plastic Surgery School, University of Milan, Milan, Italy;Department of Medical Biotechnology and Translational Medicine BIOMETRA, University of Milan, Milan, Italy;Laboratory of Cellular and Molecular Endocrinology, Humanitas Clinical and Research Center, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Rozzano, Italy;Plastic Surgery Unit, Clinica San Carlo, Paderno Dugnano, Italy;Plastic Surgery Unit, Humanitas Clinical and Research Center, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Rozzano, Italy;

关键词: adipose-derived stem cells;    cell therapies;    tamoxifen;    autologous fat grafting;    breast cancer;   

DOI  :  10.3389/fcell.2021.555248

来源: DOAJ

【 摘 要 】

Autologous fat grafting (AFG) is a safe and minimally invasive procedure to correct soft tissue defects. The benefit of AFG is attributed to adipose-derived stem cells (ASCs) in fat tissue graft. This technique is useful also in patients undergoing reconstructive surgery following quadrantectomy for breast cancer. However, these patients are frequently treated with tamoxifen. We evaluated the ex vivo effects of tamoxifen on ASCs to understand if cellular functions of ASCs are affected. We selected 24 female patients; 10 of which were breast cancer patients treated with quadrantectomy and tamoxifen. As control group, we selected 14 healthy female subjects (9 premenopausal and 5 menopausal). We found that tamoxifen has no effect on cellular proliferation, VEGF secretion or apoptosis of ASCs. The gene expression assessment demonstrated no impairment in differentiation capacity of ASCs. Our results showed that tamoxifen has no effect on cellular functions of ASCs for the first time in an ex vivo single-center study.

【 授权许可】

Unknown