Polymyxin Resistant Bacteria in Australian Poultry

Frontiers in Sustainable Food Systems

Muhd Haziq F. Abdul Momin¹  David W. Wareham²  David C. Bean³  Sarah M. Wigmore³ 

[1] Antimicrobial Research Group, Centre for Immunobiology, Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom;Division of Infection, Barts Healthcare National Health Service (NHS) Trust, London, United Kingdom;School of Science, Psychology and Sport, Federation University Australia, Ballarat, VIC, Australia;

关键词: Aeromonas;    polymyxin-resistance;    colistin;    poultry;    Hafnia paralvei;    antibiotics;   

DOI  :  10.3389/fsufs.2020.550318

来源: DOAJ

【 摘 要 】

Resistance to last-resort antibiotics is significant public health issue. Antibiotic use in animal husbandry may be a driver of resistance that can subsequently be disseminated via the food chain. This study sought to determine the level of polymyxin resistance in Gram-negative pathogens present in Australian poultry, particularly the presence of mobilizable mechanisms of polymyxin resistance. Cloacal swabs from 213 birds were taken in a point prevalence survey from six different farms at a Victorian chicken processing facility. Colistin resistant organisms were recovered by direct plating on CHROMagar COL-APSE media. Bacterial isolates were identified and analyzed by MALDI-TOF, biochemical and genotypic assays. The 213 specimens yielded 57 (26.8%) colistin-resistant Gram-negative organisms, all of which have been previously described as exhibiting intrinsic resistance to polymyxin antibiotics. The most frequent organism was identified as Hafnia paralvei (40/57; 70%). Other colistin-resistant organisms included Aeromonas hydrophila (16%), Myroides odoratus (7%), Alcaligenes faecalis (5%), and Pseudochrobactrum spp. (2%). No mobile colistin resistance (mcr) genes were detected, although the arnA gene was identified in two A. hydrophila isolates and may mediate colistin resistance in these isolates. Intrinsic polymyxin-resistant organisms are widely distributed in the food chain, with over a quarter of the birds tested yielding a polymyxin-resistant organism. However, strains containing mcr genes remain rare in Australian poultry.

【 授权许可】

Unknown