Identification of Uterine Microbiota in Infertile Women Receiving in vitro Fertilization With and Without Chronic Endometritis

Frontiers in Cell and Developmental Biology

Buzhen Tan¹  Xia He²  Kehong Wei²  Tingtao Chen²  Jing Wei³  Lin Li⁴  Weijun Chen⁴  Lijuan Yang⁴ 

[1] Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanchang University, Nanchang, China;Institute of Translational Medicine, Nanchang University, Nanchang, China;School of Life Sciences, Nanchang University, Nanchang, China;The Reproductive Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang, China;

关键词: uterine microbiota;    in vitro fertilization;    infertility;    chronic endometritis;    high-throughput sequencing;   

DOI  :  10.3389/fcell.2021.693267

来源: DOAJ

【 摘 要 】

In vitro fertilization (IVF) is an important assisted reproductive technology in treating infertility, whose failure rate is still high. Studies suggested that uterine microbiota are related to women’s reproductive diseases and persisting intrauterine bacterial infectious conditions, such as chronic endometritis (CE), impairing the pregnant processes. However, the relationship between uterine microbiota and IVF outcomes is still an open question. In the present study, 94 patients diagnosed with infertility were enrolled and were divided into CE (E group, n = 25) and non-CE (NE group, n = 69) groups depending on the hysteroscopy and immunohistochemistry. Subsequently, E (Ep, n = 8 and Enp, n = 17) and NE (NEp, n = 41 and NEnp, n = 28) groups were divided into pregnancy and non-pregnancy groups depending on the IVF outcomes, respectively. The uterine fluids were collected and microbial profiles were examined through the V4 region of 16S rRNA gene high-throughput sequencing. The results demonstrated that patients with CE had significantly lower clinical pregnancy rate compared with the non-CE patients (32 vs. 58.42%, p = 0.0014). The relative abundances of Proteobacteria and Acidobacteria were higher in the non-CE group, whereas high abundances of Actinobacteria and Fusobacteria were observed in the CE group at the phylum level. At the genus level, high relative abundances of Gardnerella were observed in the CE group and non-pregnancy groups, which significantly referred to the negative IVF outcome. In conclusion, CE may be a key factor for the negative outcome after IVF, of which the uterine microbiota plays a pivotal role, and the microbial diversity in uterine may serve as a biomarker to forecast the success of IVF outcome.

【 授权许可】

Unknown