Tracking viral particles in the intestinal contents of the American bullfrog, Lithobates catesbeianus, by Transmission Electron Microscopy

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

N.A. Melo¹  M. Hipolito¹  M.H. Catroxo¹  P.C. Teixeira²  C.M. Ferreira²  F.M. França²  R.M. Takemoto³  A.M. Antonucci³ 

[1] Instituto Biológico do Estado de São Paulo;Instituto de Pesca, APTA, SAA;Universidade Estadual de Maringá;

关键词: anfíbios;    herpesvírus;    coronavírus;    paramixovírus;    parvovírus;   

DOI  :  10.1590/1678-41626459

来源: DOAJ

【 摘 要 】

Feces are an important viral agent elimination route for infected carrier animals and in aquatic organisms these pathogenic agents can very rapidly propagate due to the habitation environment. The objective of this work is to track viral particles in the intestinal contents of bullfrogs (Lithobates catesbeianus) from five commercial frog farms in the region of Vale do Paraíba, in the State of São Paulo, Brazil, using negative contrast transmission electron microscopy (TEM). The Coronaviridae, Paramyxoviridae, Parvoviridae and Herpesviridae families were observed and photographed in specimens. This work emphasizes the importance of adopting sanitary measures in commercial farms and confirms that observing feces by TEM is an efficient and rapid diagnostic tool for detecting viral agents.

【 授权许可】

Unknown