Melioidosis presenting as a mycotic aneurysm in a Korean patient, diagnosed by 16S rRNA sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

International Journal of Infectious Diseases

Hee Ryeong Jang¹  Kye-Hyung Kim¹  Min Ji Kim¹  Jongyoun Yi²  Soon Jung Ok²  Chung Won Lee³  Mi Ju Bae³  Seunghwan Song³ 

[1] Department of Internal Medicine, Pusan National University Hospital, 179 Gudeok-ro, Seogu, Busan 602-739, Republic of Korea;Department of Laboratory Medicine, Pusan National University Hospital, Seogu, Busan, Republic of Korea;Department of Thoracic and Cardiovascular Surgery, Pusan National University Hospital, Seogu, Busan, Republic of Korea;

关键词: Burkholderia pseudomallei;    Melioidosis;    Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry;    RNA sequence analysis;   

DOI  :  10.1016/j.ijid.2015.07.012

来源: DOAJ

【 摘 要 】

A case of melioidosis with a mycotic aneurysm is reported. The blood and tissue isolates were identified as three different species of Burkholderia using the automated identification systems, VITEK 2 and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The isolate was confirmed as Burkholderia pseudomallei by 16S rRNA sequencing. The typical features of the Gram staining of colonies and 16S rRNA sequencing can be useful to identify B. pseudomallei.

【 授权许可】

Unknown