Importance of the Host Phenotype on the Preservation of the Genetic Diversity in Codling Moth Granulovirus

Viruses

Myriam Siegwart¹  Christine Blachere-López²  Miguel López-Ferber²  Benoit Graillot²  Samantha Besse³ 

[1] INRA, unité PSH, Agroparc, CEDEX 9, 84914 Avignon, France;LGEI, Ecole des Mines d’Alès, Institut Mines-Telecom et Université de Montpellier Sud de France, 6 Avenue de Clavières, 30319 Alès, France;Natural Plant Protection, Arysta LifeScience group, Avenue Léon Blum, 64000 Pau, France;

关键词: Cydia pomonella granulovirus;    codling moth;    biological control;    genetic diversity;    coevolution;    selection pressure;   

DOI  :  10.3390/v11070621

来源: DOAJ

【 摘 要 】

To test the importance of the host genotype in maintaining virus genetic diversity, five experimental populations were constructed by mixing two Cydia pomonella granulovirus isolates, the Mexican isolate CpGV-M and the CpGV-R5, in ratios of 99% M + 1% R, 95% M + 5% R, 90% M + 10% R, 50% M + 50% R, and 10% M + 90% R. CpGV-M and CpGV-R5 differ in their ability to replicate in codling moth larvae carrying the type I resistance. This ability is associated with a genetic marker located in the virus pe38 gene. Six successive cycles of replication were carried out with each virus population on a fully-permissive codling moth colony (CpNPP), as well as on a host colony (R_GV) that carries the type I resistance, and thus blocks CpGV-M replication. The infectivity of offspring viruses was tested on both hosts. Replication on the CpNPP leads to virus lineages preserving the pe38 markers characteristic of both isolates, while replication on the R_GV colony drastically reduces the frequency of the CpGV-M pe38 marker. Virus progeny obtained after replication on CpNPP show consistently higher pathogenicity than that of progeny viruses obtained by replication on R_GV, independently of the host used for testing.

【 授权许可】

Unknown