Genome-Wide Transcriptome Profiling, Characterization, and Functional Identification of NAC Transcription Factors in Sorghum under Salt Stress

Antioxidants

Mohamed A. El-Sheikh¹  Anju Rani²  Jayanti Tokas²  Himani Punia²  Saleh Alansi³  Shikha Yashveer⁴  Sonali Sangwan⁴  Anurag Malik⁵  Maha J. Hashim⁶ 

[1] Botany and Microbiology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia;Department of Biochemistry, College of Basic Sciences & Humanities, CCS Haryana Agricultural University, Hisar 125 004, Haryana, India;Department of Biology, IBB University, Ibb, Yemen;Department of Molecular Biology, Biotechnology & Bioinformatics, College of Basic Sciences & Humanities, CCS Haryana Agricultural University, Hisar 125 004, Haryana, India;Department of Seed Science & Technology, College of Agriculture, CCS Haryana Agricultural University, Hisar 125 004, Haryana, India;School of Life Sciences, Medical School (E Floor), Queens Medical Centre, Nottingham NG7 2UH, UK;

关键词: differential gene expression;    genome-wide association;    gene ontology;    transcription factors;    sorghum;    salinity;   

DOI  :  10.3390/antiox10101605

来源: DOAJ

【 摘 要 】

Salinity stress has become a significant concern to global food security. Revealing the mechanisms that enable plants to survive under salinity has immense significance. Sorghum has increasingly attracted researchers interested in understanding the survival and adaptation strategies to high salinity. However, systematic analysis of the DEGs (differentially expressed genes) and their relative expression has not been reported in sorghum under salt stress. The de novo transcriptomic analysis of sorghum under different salinity levels from 60 to 120 mM NaCl was generated using Illumina HiSeq. Approximately 323.49 million high-quality reads, with an average contig length of 1145 bp, were assembled de novo. On average, 62% of unigenes were functionally annotated to known proteins. These DEGs were mainly involved in several important metabolic processes, such as carbohydrate and lipid metabolism, cell wall biogenesis, photosynthesis, and hormone signaling. SSG 59-3 alleviated the adverse effects of salinity by suppressing oxidative stress (H₂O₂) and stimulating enzymatic and non-enzymatic antioxidant activities (SOD, APX, CAT, APX, POX, GR, GSH, ASC, proline, and GB), as well as protecting cell membrane integrity (MDA and electrolyte leakage). Significant up-regulation of transcripts encoding the NAC, MYB, and WRYK families, NHX transporters, the aquaporin protein family, photosynthetic genes, antioxidants, and compatible osmolyte proteins were observed. The tolerant line (SSG 59-3) engaged highly efficient machinery in response to elevated salinity, especially during the transport and influx of K⁺ ions, signal transduction, and osmotic homeostasis. Our data provide insights into the evolution of the NAC TFs gene family and further support the hypothesis that these genes are essential for plant responses to salinity. The findings may provide a molecular foundation for further exploring the potential functions of NAC TFs in developing salt-resistant sorghum lines.

【 授权许可】

Unknown