Mitogen-Activated Protein Kinase 4-Regulated Metabolic Networks

International Journal of Molecular Sciences

Aneirin Alan Lott¹  Sixue Chen¹  Chuwei Lin¹  Wei Zhu¹  Craig P. Dufresne² 

[1]Department of Biology, University of Florida, Gainesville, FL 32611, USA

[2]Thermo Fisher Scientific, 1400 Northpoint Parkway, West Palm Beach, FL 33407, USA

关键词: Arabidopsis thaliana;    mitogen-activated protein kinase 4;    metabolomics and proteomics;    immunity;    cytokinesis;    polyamine metabolic networks;   

DOI  :  10.3390/ijms23020880

来源: DOAJ

【 摘 要 】

Mitogen-activated protein kinase 4 (MPK4) was first identified as a negative regulator of systemic acquired resistance. It is also an important kinase involved in many other biological processes in plants, including cytokinesis, reproduction, and photosynthesis. Arabidopsis thaliana mpk4 mutant is dwarf and sterile. Previous omics studies including genomics, transcriptomics, and proteomics have revealed new functions of MPK4 in different biological processes. However, due to challenges in metabolomics, no study has touched upon the metabolomic profiles of the mpk4 mutant. What metabolites and metabolic pathways are potentially regulated by MPK4 are not known. Metabolites are crucial components of plants, and they play important roles in plant growth and development, signaling, and defense. Here we used targeted and untargeted metabolomics to profile metabolites in the wild type and the mpk4 mutant. We found that in addition to the jasmonic acid and salicylic acid pathways, MPK4 is involved in polyamine synthesis and photosynthesis. In addition, we also conducted label-free proteomics of the two genotypes. The integration of metabolomics and proteomics data allows for an insight into the metabolomic networks that are potentially regulated by MPK4.

【 授权许可】

Unknown