Profiling of the Bacterial Microbiota along the Murine Alimentary Tract

International Journal of Molecular Sciences

Ramiro Vilchez-Vargas¹  Alexander Link¹  Martin Zenker²  Denny Schanze²  Werner Hoffmann³  Franz Salm³  Katharina Haupenthal³  Eva B. Znalesniak³ 

[1] Department of Gastroenterology, Hepatology, and Infectiology, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, 39120 Magdeburg, Germany;Institute of Human Genetics, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, 39120 Magdeburg, Germany;Institute of Molecular Biology and Medicinal Chemistry, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, 39120 Magdeburg, Germany;

关键词: microbiota;    gut bacteria;    gut microbiome;    trefoil factor;    TFF3;    mucus;   

DOI  :  10.3390/ijms23031783

来源: DOAJ

【 摘 要 】

Here, the spatial distribution of the bacterial flora along the murine alimentary tract was evaluated using high throughput sequencing in wild-type and Tff3-deficient (Tff3^KO) animals. Loss of Tff3 was linked to increased dextran sodium sulfate-induced colitis. This systematic study shows the results of 13 different regions from the esophagus to the rectum. The number of bacterial species (richness) increased from the esophagus to the rectum, from 50 to 200, respectively. Additionally, the bacterial community structure changed continuously; the highest changes were between the upper/middle and lower gastrointestinal compartments when comparing adjacent regions. Lactobacillus was the major colonizer in the upper/middle gastrointestinal tract, especially in the esophagus and stomach. From the caecum, a drastic diminution of Lactobacillus occurred, while members of Lachnospiraceae significantly increased. A significant change occurred in the bacterial community between the ascending and the transverse colon with Bacteroidetes being the major colonizers with relative constant abundance until the rectum. Interestingly, wild-type and Tff3^KO animals did not show significant differences in their bacterial communities, suggesting that Tff3 is not involved in alterations of intraluminal or adhesive microbiota but is obviously important for mucosal protection, e.g., of the sensitive stem cells in the colonic crypts probably by a mucus plume.

【 授权许可】

Unknown