The Cryopreserved Sperm Traits of Various Ram Breeds: Towards Biodiversity Conservation

Animals

Hana Ďúranová¹  Peter Chrenek²  Jakub Vozaf²  Jaromír Vašíček²  Andrea Svoradová³  Alexander V. Makarevich³  Lucia Olexiková³  Andrej Baláži³  Rastislav Jurčík³  Linda Dujíčková³ 

[1] AgroBioTech Research Centre, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 94901 Nitra, Slovakia;Institute of Biotechnology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 94901 Nitra, Slovakia;NPPC, Research Institute for Animal Production Nitra, Hlohovecka 2, 95141 Lužianky, Slovakia;

关键词: ram;    sperm;    cryopreservation;    gene bank;    biodiversity;   

DOI  :  10.3390/ani12101311

来源: DOAJ

【 摘 要 】

The aim of our research was to compare three Slovak sheep breeds in the quality parameters of cryopreserved sperm. The ejaculates of Slovak Dairy (SD), Native Wallachian (NW), and Improved Wallachian (IW) sheep rams (n = 12) were collected by electro-ejaculation. Heterospermic samples were created from suitable ejaculates, separately for each breed (at least 90% of total and 80% of progressive motility). Samples were equilibrated in a Triladyl^® diluent and frozen by automated freezing. Sperm samples were subjected to the motility, morphology, (CASA), viability and apoptosis (DRAQ7/Yo-Pro-1), fertilizing capability (penetration/fertilization test (P/F) in vitro) and acrosomal status (transmission electron microscopy) assays before freezing and after thawing. It was found that there were no significant differences (p < 0.05) between the evaluated breeds in motility, viability, apoptosis, morphological properties, and fertilizing ability of cryopreserved sperm. Significant differences occurred in acrosomal status. Our results demonstrate that the use of the selected cryopreservation protocol is suitable for at least three different sheep breeds, which can greatly benefit the biodiversity protection and simplifies the creation of an animal genetic resources gene bank.

【 授权许可】

Unknown