Rapid Detection of Mycoplasma bovis, Staphylococcus aureus and Streptococcus agalactiae in Cattle Bulk Tank Milk in Cyprus and Relations with Somatic Cell Counts

Pathogens

Antonis Antoniou¹  Costas Arsenoglou²  Christodoulos Pipis²  George Botsaris³  Nikolas Markantonis³  Maria Liapi³  Christodoulos Michael³ 

[1] Cyprus Institute of Neurology and Genetics, Nicosia 1683, Cyprus;Cyprus Veterinary Services, 1417 Athalassas Av, Nicosia 1417, Cyprus;Department of Agricultural Sciences, Biotechnology and Food Science, Cyprus University of Technology, Limassol 3036, Cyprus;

关键词: Bovine mastitis;    Mycoplasma bovis;    Staphylococcus aureus;    Streptococcus agalactiae;    somatic cells;   

DOI  :  10.3390/pathogens10070841

来源: DOAJ

【 摘 要 】

One hundred and seventy-seven (177) bulk tank milk samples were analyzed with a commercially available real-time polymerase chain reaction kit and 11 (6.21%), 41 (23.16%), and 58 (32.77%) tested positive for Mycoplasma bovis, Staphylococcus aureus, and Streptococcus agalactiae, respectively. Statistical analysis revealed a significant relationship between the presence of S. aureus and S. agalactiae. Enumeration of somatic cells was performed in the same samples by flow cytometry. The somatic cell counts were found higher in S. aureus and S. agalactiae positive samples. No association was found between M. bovis presence and somatic cells counts. Low internal assay control Ct values were found to be related with high somatic cell counts. Noticeably, this is the first report for the presence of M. bovis in Cyprus. Therefore, its presence was confirmed by bulk tank milk culture, conventional PCR, and next generation sequencing. Furthermore, M. bovis was typed with multilocus sequencing typing and was allocated to sequence type 29 (ST 29). Real-time PCR in bulk tank milk samples is a useful tool to detect mammary infections, especially for neglected pathogens such as M. bovis.

【 授权许可】

Unknown