The effectivity of topical mangosteen pericarp extract cream on wound healing in Swiss albino mice

Journal of Biological Research

Upik A. Miskad¹  Hilda Brigitta Sombolayuk²  Khairuddin Djawad²  Siswanto Wahab²  Gemini Alam³  Ilhamjaya Pattelongi⁴ 

[1] Department of Anatomical Pathology, Faculty of Medicine, Hasanuddin University, Makassar;Department of Dermatology and Venereology, Faculty of Medicine, Hasanuddin University, Makassar;Department of Pharmacognosy, Faculty of Pharmacy, Hasanuddin University, Makassar;Department of Physiology, Faculty of Medicine, Hasanuddin University, Makassar;

关键词: Anti-inflammation;    Granulation tissue formation;    Mangosteen pericarp extract cream;    Reepithelialization;    Wound healing;   

DOI  :  10.4081/jbr.2019.8150

来源: DOAJ

【 摘 要 】

Wound healing is a complex physiological process consisting of four phases: coagulation, inflammation, proliferation and migration, and remodeling, each with distinct characteristics. Studies have suggested that mangosteen pericarp extract (MPE) may accelerate wound healing. However, the mechanism is not fully understood. This study aims to evaluate the effect of MPE cream in various concentrations pericarp in acute wound healing of albino mice, both histologically and macroscopically. Thirty-two healthy female Swiss albino mice, aged 6-9 weeks, weight 20-30 g, were included in this study. The samples were randomly divided into eight groups each consisting of 4 mice. The first four groups were treated with MPE cream 5%, 10%, and 20%, and a control group that was given no medication, respectively, and were sacrificed after three days. The other four groups received the same application and were sacrificed after 8 days. Wound bed diameter was measured and biopsy from the skin lesion was performed for histopathologic examination. Mann-Whitney test was used to analyze the diameter of the wound bed and histopathological findings of granulation tissue formation, reepithelialization, and inflammation, with P<0.05 considered as significant. MPE cream significantly improved wound healing by increasing granulation tissue formation, and reepithelialization. In addition, MPE cream application was also shown to decrease the number of inflammatory cells, particularly in 5% and 10% concentrations, both in the 3-day and 8-day groups. MPE cream application can accelerate wound healing and thus it can be used in acute wound treatment.

【 授权许可】

Unknown