Screening Vitis Genotypes for Responses to Botrytis cinerea and Evaluation of Antioxidant Enzymes, Reactive Oxygen Species and Jasmonic Acid in Resistant and Susceptible Hosts

Molecules

Muhammad Hanif¹  Xiping Wang¹  Xiaoqing Hou¹  Ran Wan²  MatiUr Rahman²  Bilal Ahmad² 

[1] F University, Yangling 712100, China;;State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&

关键词: biotic stress;    Botrytis cinerea;    grape;    resistant genotypes;    reactive oxygen species;    jasmonic acid;    antioxidant enzymes;   

DOI  :  10.3390/molecules24010005

来源: DOAJ

【 摘 要 】

Botrytis cinerea is a necrotrophic fungal phytopathogen with devastating effects on many Vitis genotypes. Here, a screening of 81 Vitis genotypes for leaf resistance to B. cinerea revealed two highly resistant (HR), twelve resistant (R), twenty-five susceptible (S) and forty-two highly susceptible (HS) genotypes. We focused on the HR genotype, ‘Zi Qiu’ (Vitis davidii), and the HS genotype ‘Riesling’ (V. vinifera), to elucidate mechanisms of host resistance and susceptibility against B. cinerea, using detached leaf assays. These involved a comparison of fungal growth, reactive oxygen species (ROS) responses, jasmonic acid (JA) levels, and changes in the anti-oxidative system between the two genotypes after inoculation with B. cinerea. Our results indicated that the high-level resistance of ‘Zi Qiu’ can be attributed to insignificant fungal development, low ROS production, timely elevation of anti-oxidative functions, and high JA levels. Moreover, severe fungal infection of ‘Riesling’ and sustained ROS production coincided with relatively unchanged anti-oxidative activity, as well as low JA levels. This study provides insights into B. cinerea infection in grape, which can be valuable for breeders by providing information for selecting suitable germplasm with enhanced disease resistance.

【 授权许可】

Unknown