Screening of Onosma Species for Cytotoxic Activity

Proceedings

Seda Duman¹  Erdal Bedir¹  Özge Güzel¹  Ademi Fahri Pirhan²  Sinem Yılmaz³ 

[1] Department of Bioengineering, Faculty of Engineering, Izmir Institute of Technology, Izmir 35000, Turkey;Department of Biology, Faculty of Science, Ege University, Izmir 35000, Turkey;Department of Biotechnology, Institute of Science, Ege University, Izmir 35000, Turkey;

关键词: Onosma;    endemic;    extraction;    cytotoxic activity;    in vitro;    MTT;   

DOI  :  10.3390/proceedings1101048

来源: DOAJ

【 摘 要 】

Onosma L. genus (Boraginaceae) comprises of 230 species that is represented by 102 species in the flora of Turkey with almost 50% endemism ratio (48 species), implying Anatolia as the gene center of Onosma species. In this study, we evaluated the petroleum ether, dichlorometane and methanol root extracts of 5 Onosma species (O. mite, O. mollis, O. taurica var. taurica, O. frutescens, and an unidentified Onosma member) collected from Southwestern Turkey for their cytotoxic activities. The extracts were screened versus seven human cancer cell lines, namely DU145, Capan-1, HCC-1937, MCF-7, HeLa, HEPG2, A-459 and a normal cell line, MRC-5, using MTT assay. As a result, the petroleum ether and dichlorometane extracts of Onosma species, rich in naphthoquinones, were more active than the methanol extracts in a dose range of 8 to 32 μg/mL. The petroleum ether and dichlorometane extracts of O. taurica and the unidentified species along with the petroleum ether extract of O. mollis showed strong cytotoxicity versus all the cell lines with IC50 values lower than 8 μg/mL (the lowest test dose). Thus, O. taurica var. taurica and O. mollis were taken into cytotoxic activity-guided fractionation studies to isolate the bioactive compounds.

【 授权许可】

Unknown