Prevalence and molecular identification of Cryptosporidium spp in cattle in Baghdad province, Iraq

Iraqi Journal of Veterinary Sciences

M.H. Kawan¹  H.H. Alseady¹ 

[1] Department of Parasitology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq;

关键词: cryptosporidium;    prevalence;    cattle;    nested pcr;    sequences analysis;   

DOI  :  10.33899/ijvs.2019.163084

来源: DOAJ

【 摘 要 】

The objective of this study was to record the prevalence rate of Cryptosporidium and to determine the Cryptosporidium spp in cattle in different parts of Baghdad province. A total number of 100 fecal samples from different age groups were collected. Conventional method using modified Ziehl-Neelsen for staining fecal smears and molecular techniques for detection the prevalence and determines the species of Cryptosporidium that cause infection. The overall prevalence of infection with Cryptosporidium was 21% (21/100) by conventional method; nested PCR was done that targeting 18S rRNA gene on the same samples in which Cryptosporidium DNA identified in 38 samples (38%). Four species of Cryptosporidium in cattle were detected for the first time in Baghdad province: C. parvum (6/10), C. andersoni (2/10), C. bovis (1/10) and C. ryanae (1/10). The determination and characterization of Cryptosporidium spp in cattle was very important to avoid the infection to other animals and handlers and for applying control programs.

【 授权许可】

Unknown