Valorization of Fermented Shrimp Waste with Supercritical CO2 Conditions: Extraction of Astaxanthin and Effect of Simulated Gastrointestinal Digestion on Its Antioxidant Capacity

Molecules

Gustavo Adolfo González-Aguilar¹  Pedro de Jesús Bastidas-Bastidas²  José Basilio Heredia²  Luis Angel Cabanillas-Bojórquez²  Leticia Xochitl López-Martinez³  Erick Paul Gutiérrez-Grijalva⁴  Ramón Ignacio Castillo-López⁵ 

[1] Centro de Investigación en Alimentación y Desarrollo, A. C. CTAOV, Carretera Gustavo Enrique Astiazarán Rosas, No. 46, Col. La Victoria, Hermosillo CP 83304, Sonora, Mexico;Centro de Investigación en Alimentación y Desarrollo, A. C. Carretera a Eldorado Km 5.5 Col. Campo El Diez, Culiacán CP 80110, Sinaloa, Mexico;Cátedras CONACyT-Centro de Investigación en Alimentación y Desarrollo, A. C. Carretera Gustavo Enrique Astiazarán Rosas, No. 46, Col. La Victoria, Hermosillo CP 83304, Sonora, Mexico;Cátedras CONACyT-Centro de Investigación en Alimentación y Desarrollo, A. C. Carretera a Eldorado Km 5.5 Col. Campo El Diez, Culiacán CP 80110, Sinaloa, Mexico;Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, Ciudad Universitaria, Culiacán CP 80013, Sinaloa, Mexico;

关键词: astaxanthin;    shrimp waste;    supercritical CO₂ extraction;    antioxidant activity;    bioaccessibility;    optimization;   

DOI  :  10.3390/molecules26154465

来源: DOAJ

【 摘 要 】

Lactic acid fermentation increases the bioactive properties of shrimp waste. Astaxanthin is the principal carotenoid present in shrimp waste, which can be found esterified in the liquid fraction (liquor) after its lactic acid fermentation. Supercritical CO₂ technology has been proposed as a green alternative to obtain astaxanthin from fermented shrimp waste. This study aimed to optimize astaxanthin extraction by supercritical CO₂ technology from fermented liquor of shrimp waste and study bioaccessibility using simulated gastrointestinal digestion (GD) of the optimized extract. A Box–Behnken design with three variables (pressure, temperature, and flow rate) was used to optimize the supercritical CO₂ extraction. The optimized CO₂ extract was obtained at 300 bar, 60 °C, and 6 mL/min, and the estimated characteristics showed a predictive extraction yield of 11.17%, antioxidant capacity of 1.965 mmol of Trolox equivalent (TE)/g, and astaxanthin concentration of 0.6353 µg/g. The experiment with optimal conditions performed to validate the predicted values showed an extraction yield of 12.62%, an antioxidant capacity of 1.784 mmol TE/g, and an astaxanthin concentration of 0.52 µg/g. The astaxanthin concentration decreased, and the antioxidant capacity of the optimized extract increased during gastrointestinal digestion. In conclusion, our optimized supercritical CO₂ process is suitable for obtaining astaxanthin from shrimp by-products after lactic acid fermentation.

【 授权许可】

Unknown