Chemogenetic activation of nigrostriatal dopamine neurons in freely moving common marmosets

iScience

Takafumi Minamimoto¹  Takashi Okauchi¹  Tetsuya Suhara²  Yukiko Hori²  Chika Sato³  Noriaki Yahata³  Koki Mimura³  Hisao Nishijo³  Yuji Nagai³  Jumpei Matsumoto⁴  Masahiko Takada⁴  Makoto Higuchi⁵  Kei Kimura⁵  Ken-ichi Inoue⁶  Toshiyuki Hirabayashi⁶ 

[1] Applied MRI Research, Department of Molecular Imaging and Theranostics, National Institutes for Quantum and Radiological Science and Technology, Chiba 263-8555 Japan;Research Center for Idling Brain Science, University of Toyama, Toyama 930-8555, Japan;Department of Functional Brain Imaging, National Institutes for Quantum and Radiological Science and Technology, Chiba 263-8555 Japan;Department of System Emotional Science, Faculty of Medicine, University of Toyama, Toyama 930-8555, Japan;Quantum Life Informatics Group, Institute for Quantum Life Science, National Institutes for Quantum and Radiological Science and Technology, Chiba 263-8555 Japan;Systems Neuroscience Section, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan;

关键词: behavioral neuroscience;    molecular neuroscience;    cellular neuroscience;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: To interrogate particular neuronal pathways in nonhuman primates under natural and stress-free conditions, we applied designer receptors exclusively activated by designer drugs (DREADDs) technology to common marmosets. We injected adeno-associated virus vectors expressing the excitatory DREADD hM3Dq into the unilateral substantia nigra (SN) in four marmosets. Using multi-tracer positron emission tomography imaging, we detected DREADD expression in vivo, which was confirmed in nigrostriatal dopamine neurons by immunohistochemistry, as well as by assessed activation of the SN following agonist administration. The marmosets rotated in a contralateral direction relative to the activated side 30–90 min after consuming food containing the highly potent DREADD agonist deschloroclozapine (DCZ) but not on the following days without DCZ. These results indicate that non-invasive and reversible DREADD manipulation will extend the utility of marmosets as a primate model for linking neuronal activity and natural behavior in various contexts.

【 授权许可】

Unknown