Identification of Prognostic miRNAs Associated With Immune Cell Tumor Infiltration Predictive of Clinical Outcomes in Patients With Non-Small Cell Lung Cancer

Frontiers in Oncology

Shoude Jin¹  Kai Mi²  Zhiheng Li³  Lixia Qiang⁴  Meiyu Lv⁴  Yushan Wu⁴  Yuepeng Zhang⁴  Ligong Yuan⁵ 

[1] Peking Union Medical College, Beijing, China;College of Bioinformatics Science and Technology, Harbin Medical University, Harbin, China;Department of Medical Oncology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, China;Department of Respiratory, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, China;;Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences &

关键词: NSCLC;    immune infiltration;    miRNAs;    prognosis;    miRNA-target network;   

DOI  :  10.3389/fonc.2021.705869

来源: DOAJ

【 摘 要 】

BackgroundA detailed means of prognostic stratification in patients with non-small cell lung cancer (NSCLC) is urgently needed to support individualized treatment plans. Recently, microRNAs (miRNAs) have been used as biomarkers due to their previously reported prognostic roles in cancer. This study aimed to construct an immune-related miRNA signature that effectively predicts NSCLC patient prognosis.MethodsThe miRNAs and mRNA expression and mutation data of NSCLC was obtained from The Cancer Genome Atlas (TCGA). Immune-associated miRNAs were identified using immune scores calculated by the ESTIMATE algorithm. LASSO-penalized multivariate survival models were using for development of a tumor immune-related miRNA signature (TIM-Sig), which was evaluated in several public cohorts from the Gene Expression Omnibus (GEO) and the CellMiner database. The miRTarBase was used for constructing the miRNA-target interactions.ResultsThe TIM-Sig, including 10 immune-related miRNAs, was constructed and successfully predicted overall survival (OS) in the validation cohorts. TIM-Sig score negatively correlated with CD8+ T cell infiltration, IFN-γ expression, CYT activity, and tumor mutation burden. The correlation between TIM-Sig score and genomic mutation and cancer chemotherapeutics was also evaluated. A miRNA-target network of 10 miRNAs in TIM-Sig was constructed. Further analysis revealed that these target genes showed prognostic value in both lung squamous cell carcinoma and adenocarcinoma.ConclusionsWe concluded that the immune-related miRNAs demonstrated a potential value in clinical prognosis.

【 授权许可】

Unknown