Enhancement of Virus Infection Using Dynamic Cell Culture in a Microchannel

Micromachines

HeeKyung Jin¹  Jae-sung Bae²  GyuMan Kim³  JeongA Kim³  ChulMin Kim³  HyeJin Choi³ 

[1] Department of Laboratory Animal Medicine, College of Veterinary Medicine, Kyungpook National University, 80 Daehakro, Buk-gu, Daegu 41566, Korea;Department of Physiology, School of Medicine, Kyungpook National University, 680 Gukchaebosang-ro, Jung-Gu, Daegu 41944, Korea;School of Mechanical Engineering, Kyungpook National University, 80 Daehakro, Buk-gu, Daegu 41566, Korea;

关键词: virus infection;    microchannel;    dynamic cell culture;    induced pluripotent stem cells (iPSCs);   

DOI  :  10.3390/mi9100482

来源: DOAJ

【 摘 要 】

With increasing interest in induced pluripotent stem cells (iPSCs) in the field of stem cell research, highly efficient infection of somatic cells with virus factors is gaining importance. This paper presents a method of employing microfluidic devices for dynamic cell culture and virus infection in a microchannel. The closed space in the microchannel provided a better environment for viruses to diffuse and contact cell surfaces to infect cells. The microfluidic devices were fabricated by photolithography and soft lithography. NIH/3T3 fibroblast cells were cultured in the microfluidic device in static and dynamic conditions and compared with the conventional culture method of using Petri dishes. Virus infection was evaluated using an enhanced green fluorescent protein virus as a model. Dynamic culture in the microchannel showed similar growth of cells to that in Petri dish culture, but the virus infection efficiency was four-times higher. The proposed dynamic culture system could be useful in iPSC research by providing efficient virus infection tools.

【 授权许可】

Unknown