Chemometrics-Assisted Identification of Anti-Inflammatory Compounds from the Green Alga Klebsormidium flaccidum var. zivo

Molecules

Jianping Zhao¹  IkhlasA. Khan¹  Shi Qiu¹  Siyu Ren¹  Xing-Cong Li¹  Mei Wang¹  ShabanaI. Khan¹  WilliamP. Pfund²  Amy Steffek² 

[1] National Center for Natural Product Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, The University of Mississippi, Oxford, MS 38677, USA;ZIVO Biosciences, INC, 2804 Orchard Lake Road, Suite 202, Keego Harbor, MI 48320, USA;

关键词: klebsormidium flaccidum var. zivo;    klebsormidiaceae;    anti-inflammatory;    inos;    nf-κb;    chemometrics;   

DOI  :  10.3390/molecules25051048

来源: DOAJ

【 摘 要 】

The green alga Klebsormidium flaccidum var. zivo is a rich source of proteins, polyphenols, and bioactive small-molecule compounds. An approach involving chromatographic fractionation, anti-inflammatory activity testing, ultrahigh performance liquid chromatography-mass spectrometry profiling, chemometric analysis, and subsequent MS-oriented isolation was employed to rapidly identify its small-molecule anti-inflammatory compounds including hydroxylated fatty acids, chlorophyll-derived pheophorbides, carotenoids, and glycoglycerolipids. Pheophorbide a, which decreased intracellular nitric oxide production by inhibiting inducible nitric oxide synthase, was the most potent compound identified with an IC₅₀ value of 0.24 µM in lipopolysaccharides-induced macrophages. It also inhibited nuclear factor kappaB activation with an IC₅₀ value of 32.1 µM in phorbol 12-myristate 13-acetate-induced chondrocytes. Compared to conventional bioassay-guided fractionation, this approach is more efficient for rapid identification of multiple chemical classes of bioactive compounds from a complex natural product mixture.

【 授权许可】

Unknown