Synthesis of Natural Nano-Hydroxyapatite from Snail Shells and Its Biological Activity: Antimicrobial, Antibiofilm, and Biocompatibility

Membranes

Sameer H. Qari¹  Areej Suliman Al-Meshal²  Eman Hillal Althubaiti³  Rokayya Sami⁴  Amani H. Aljahani⁵  Mahmoud Youssef⁶  Ahmed Atef⁷  Reda Shehata⁷  Nesreen Safwat⁷  Sayed Kareem⁷  Hanaa Y. Ahmed⁷ 

[1] Department of Biology, Al-Jumum University College, Umm Al-Qura University, Makkah 21955, Saudi Arabia;Department of Biology, College of Science and Humanities in Al-Kharj, Prince Sattam bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia;Department of Biotechnology, Faculty of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia;Department of Food Science and Nutrition, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia;Department of Physical Sport Science, College of Education, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia;Food Science and Technology Department, Faculty of Agriculture, Al-Azhar University, Cairo 11787, Egypt;The Regional Center for Mycology and Biotechnology, Al-Azhar University, Cairo 11787, Egypt;

关键词: snail shell;    hydroxyapatite;    nanoparticles;    antimicrobial;    antibiofilm;    biocompatibility;   

DOI  :  10.3390/membranes12040408

来源: DOAJ

【 摘 要 】

Hydroxyapatite nanoparticles (HAn) have been produced as biomaterial from biowaste, especially snail shells (Atactodea glabrata). It is critical to recycle the waste product in a biomedical application to overcome antibiotic resistance as well as biocompatibility with normal tissues. Moreover, EDX, TEM, and FT-IR analyses have been used to characterize snail shells and HAn. The particle size of HAn is about 15.22 nm. Furthermore, higher inhibitory activity was observed from HAn than the reference compounds against all tested organisms. The synthesized HAn has shown the lowest MIC values of about 7.8, 0.97, 3.9, 0.97, and 25 µg/mL for S. aureus, B. subtilis, K. pneumonia, C. albicans, and E. coli, respectively. In addition, the HAn displayed potent antibiofilm against S. aureus and B. subtilis. According to the MTT, snail shell and HAn had a minor influence on the viability of HFS-4 cells. Consequently, it could be concluded that some components of waste, such as snail shells, have economic value and can be recycled as a source of CaO to produce HAn, which is a promising candidate material for biomedical applications.

【 授权许可】

Unknown