Synergistic Effects on Incorporation of β-Tricalcium Phosphate and Graphene Oxide Nanoparticles to Silk Fibroin/Soy Protein Isolate Scaffolds for Bone Tissue Engineering

Polymers

Jia He¹  Chen Liu¹  Bowen Zheng²  Fan Liu²  Xiaohong Wang³  Jun Liu⁴  In-seop Lee⁴  Cen Chen⁴  Yi Liu⁵ 

[1] Organ Manufacturing, School of Fundamental Sciences, China Medical University (CMU), No. 77 Puhe Road, Shenyang North New Area, Shenyang 110122, China;;Center of 3D Printing &College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China;Department of Orthodontics, School of Stomatology, China Medical University, Shenyang 110122, China;Institute of Natural Sciences, Yonsei University, Seoul 120-749, Korea;

关键词: graphene oxide;    β-tricalcium phosphate;    silk fibroin;    soy protein isolate;    scaffold;    osteogenesis;   

DOI  :  10.3390/polym12010069

来源: DOAJ

【 摘 要 】

In bone tissue engineering, an ideal scaffold is required to have favorable physical, chemical (or physicochemical), and biological (or biochemical) properties to promote osteogenesis. Although silk fibroin (SF) and/or soy protein isolate (SPI) scaffolds have been widely used as an alternative to autologous and heterologous bone grafts, the poor mechanical property and insufficient osteoinductive capability has become an obstacle for their in vivo applications. Herein, β-tricalcium phosphate (β-TCP) and graphene oxide (GO) nanoparticles are incorporated into SF/SPI scaffolds simultaneously or individually. Physical and chemical properties of these composite scaffolds are evaluated using field emission scanning electron microscope (FESEM), X-ray diffraction (XRD) and attenuated total reflectance Fourier transformed infrared spectroscopy (ATR-FTIR). Biocompatibility and osteogenesis of the composite scaffolds are evaluated using bone marrow mesenchymal stem cells (BMSCs). All the composite scaffolds have a complex porous structure with proper pore sizes and porosities. Physicochemical properties of the scaffolds can be significantly increased through the incorporation of β-TCP and GO nanoparticles. Alkaline phosphatase activity (ALP) and osteogenesis-related gene expression of the BMSCs are significantly enhanced in the presence of β-TCP and GO nanoparticles. Especially, β-TCP and GO nanoparticles have a synergistic effect on promoting osteogenesis. These results suggest that the β-TCP and GO enhanced SF/SPI scaffolds are promising candidates for bone tissue regeneration.

【 授权许可】

Unknown