MiRNA Profiling in Pectoral Muscle Throughout Pre- to Post-Natal Stages of Chicken Development

Frontiers in Genetics

Shaolan Zhang¹  Mingwang Zhang²  Bo Zeng²  Jian Gao²  Shailendra Kumar Mishra²  Qing Zhu²  Qingyong Ni²  Zhongxian Xu²  Xiaoling Zhao²  Diyan Li²  Yan Wang²  Mingyao Yang²  Deying Yang²  Xiaolan Fan²  Yan Li²  Huadong Yin²  Min Chen³ 

[1] Department of Science and Technology, Chengdu Medical College, Chengdu, China;Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricltural University, Wenjiang, China;Institute of Biopharming, West China Hospital, Sichuan University, Chengdu, China;

关键词: chicken;    miRNA;    myoblast;    differentiation;    gga-miRNA-454;   

DOI  :  10.3389/fgene.2020.00570

来源: DOAJ

【 摘 要 】

MicroRNA (miRNA) is known to be an important regulator of muscle growth and development. The regulation of microRNA on the skeletal muscle phenotype of animals is mainly achieved by regulating the proliferation and differentiation of myoblasts. In this study, we sequenced a total of 60 samples from 15 developing stages of the pectoral muscle and five other tissues at 300 days of Tibetan chicken. We characterized the expression patterns of miRNAs across muscle developmental stages, and found that the chicken growth and development stage was divided into early-embryonic and late-embryonic as well as postnatal stages. We identified 81 and 21 DE-miRNAs by comparing the miRNA profiles of pectoral muscle of three broad periods and different tissues, respectively; and 271 miRNAs showed time-course patterns. Their potential targets were predicted and used for functional enrichment to understand their regulatory functions. Significantly, GgmiRNA-454 is a time-dependent and tissue-differential expression miRNA. In order to elucidate the role of gga-miRNA-454 in the differentiation of myoblasts, we cultured chicken myoblasts in vitro. The results show that although gga-miRNA-454-3p initiates increase and thereafter decrease during the chicken myoblasts differentiation, it had no effect on primary myoblasts proliferation. Furthermore, we confirm that gga-miRNA-454 inhibits myoblast differentiation by targeting the myotube-associated protein SBF2.

【 授权许可】

Unknown