INFLUENCE OF THE SEASONAL VARIATION ON THE BLASTOCYST YIELD OF C57BL/6NCrl MOUSE STRAIN

Scientific Papers Animal Science and Biotechnologies

关键词: C57Bl/6NCrl;    superovulation;    blastocysts;    seasonal variation;    chimeras;   

DOI  :  

来源: DOAJ

【 摘 要 】

Routine generation of chimeras relies on the production of large numbers ofblastocysts in a constant and reproducible manner and on the quality of the targetedES (embryonal stem) cells used for microinjection. We have focussed this work onthe production of large numbers of blastocysts by superovulation, in standardizedconditions. The efficiency of superovulation in mice is affected by several factors.These factors depend on the animal, on the environment and on the operator. Theeffect of seasonal variation as environmental impact was assessed. For this purpose360 prepubescent C57BL6/NCrl females were superovulated with 5 IU of PMSG(Pregnant Mare’s Serum Gonadotropin) and 5 IU hCG (Human ChorionicGonadotropin) and mated with fertile C57BL/6NCrl males. The distribution of theexperiments over the seasons was as following: 8 experiments were performed inspring, 12 in summer, 12 in autumn and 4 in winter. The influence of the seasonalvariation on the efficiency of superovulation in two age categories (23 day old and26 day old females ) was compared. The results show that the overall vaginal plugpositive rate (VP+%) of the 23 day old females (90%) is higher in comparison tothat of the 26 day old females (86,4%), whereas the blastocyst rate (B%) of 26 dayold females (45,4%) is higher compared to the 23 day old females (41%). Theinfluence of the seasonal variation on the yield of embryos and the rate ofblastocysts is relatively constant in each season. These results suggest that housingof the mice in our artificial conditions and the hormonal induction of superovulationlead to a reproducible number of blastocysts over the seasonal variation of the year.

【 授权许可】

Unknown