An In Vitro Lung System to Assess the Proinflammatory Hazard of Carbon Nanotube Aerosols

International Journal of Molecular Sciences

BediaBegum Karakocak¹  Alke Petri-Fink¹  Hana Barosova¹  Dedy Septiadi¹  Barbara Rothen-Rutishauser¹  Vicki Stone² 

[1] BioNanomaterials Group, Adolphe Merkle Institute, University of Fribourg, Fribourg 1700, Switzerland;Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot-Watt University, Edinburgh, EH14 4AS, UK;

关键词: lung;    in vitro;    co-culture;    carbon nanotubes;    multiwalled carbon nanotubes;    air-liquid interface;   

DOI  :  10.3390/ijms21155335

来源: DOAJ

【 摘 要 】

In vitro three-dimensional (3D) lung cell models have been thoroughly investigated in recent years and provide a reliable tool to assess the hazard associated with nanomaterials (NMs) released into the air. In this study, a 3D lung co-culture model was optimized to assess the hazard potential of multiwalled carbon nanotubes (MWCNTs), which is known to provoke inflammation and fibrosis, critical adverse outcomes linked to acute and prolonged NM exposure. The lung co-cultures were exposed to MWCNTs at the air-liquid interface (ALI) using the VITROCELL^® Cloud system while considering realistic occupational exposure doses. The co-culture model was composed of three human cell lines: alveolar epithelial cells (A549), fibroblasts (MRC-5), and macrophages (differentiated THP-1). The model was exposed to two types of MWCNTs (Mitsui-7 and Nanocyl) at different concentrations (2–10 µg/cm²) to assess the proinflammatory as well as the profibrotic responses after acute (24 h, one exposure) and prolonged (96 h, repeated exposures) exposure cycles. The results showed that acute or prolonged exposure to different concentrations of the tested MWCNTs did not induce cytotoxicity or apparent profibrotic response; however, suggested the onset of proinflammatory response.

【 授权许可】

Unknown