Molecular typing of Staphylococcus aureus isolate responsible for staphylococcal poisoning incident in homemade food

Italian Journal of Food Safety

Lucia Decastelli¹  Silvia Gallina¹  Francesco Chiesa¹  Alberto Bellio¹  Francesco Casalinuovo²  Daniela Manila Bianchi²  Guerrino Macori²  Fabio Zuccon²  Pier Luigi Acutis³  Daniela Adriano³ 

[1] Italian ReferenceLaboratory for Coagulase PositiveStaphylococci including S. aureus,Institute for Experimental VeterinaryMedicine of Piedmont, Liguria and AostaValley, Turin;Institute for Experimental VeterinaryMedicine of Piedmont, Liguria and AostaValley, Turin;Institute for Experimental Veterinary Medicine of Piedmont, Liguria and Aosta Valley, Turin;

关键词: Staphylococcus aureus;    Foodborne pathogen;    Outbreak investigation;    Molecular typing;    Spa-typing;   

DOI  :  10.4081/ijfs.2016.5736

来源: DOAJ

【 摘 要 】

In October 2012, two persons fell ill with symptoms consistent with staphylococcal food poisoning after eating home-canned tuna fish and tomatoes. Laboratory investigation detected the enterotoxins in the home-canned tuna and molecular analysis of the isolated Staphylococcus aureus confirmed it carried toxin genes. Qualitative enzyme-linked immunosorbent assay and enzime linked fluorescent assay methods and quantitative assay identified the enterotoxins in the food leftovers, specifically staphylococcal enterotoxins type A (SEA) and D (SED), respectively 0.49 and 2.04 ng/g. The laboratory results are discussed considering the relation to the fish in oil, survival and heat resistance of S. aureus, and presumptive microbial contamination due to improper handling during home-canning procedures. This is the first reported cluster of foodborne illnesses due to staphylococcal enterotoxins in tuna in Italy. In this study, we reported cases described and analysed for their spa-type. Showing a high heterogeneity of isolates, spa-type t13252 is correlated in a node of the minimum spanning tree and it has never been reported as responsible for foodborne outbreak. This case underlines the importance of risk communication and dissemination of home-canning guidelines to reduce the incidence of foodborne outbreaks caused by homemade conserves.

【 授权许可】

Unknown