Identification of multiple isoforms of glucocorticoid receptor in nasal polyps of patients with chronic rhinosinusitis

Journal of Otolaryngology - Head and Neck Surgery

Yan Zhao¹  Shiping Bao²  Kun Du³  Xiangdong Wang³  Shan Shao³  Yue Wang³  Luo Zhang³  Yuan Xu⁴  Tie Wang⁵ 

[1] Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology;Department of Otolaryngology Head and Neck Surgery, Beijing Youan Hospital, Capital Medical University;Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University;Departments of Oncology, Community Health Sciences, and Surgery, Cumming School of Medicine, and The Center for Health Informatics, University of Calgary;MIID Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary;

关键词: Nasal polyps;    Sinusitis;    Glucocorticoid receptor;    Endotypes;   

DOI  :  10.1186/s40463-022-00561-1

来源: DOAJ

【 摘 要 】

Abstract Background The conventional belief that glucocorticosteroid (GC) acts through a single brand glucocorticoid receptor (GR)α protein has changed dramatically with the discovery of multiple GR isoforms. We aimed to evaluate whether multiple GR protein isoforms are expressed in chronic rhinosinusitis with nasal polyps (CRSwNP) and whether GR protein isoform expression profiles differ between different endotypes of CRSwNP. Methods Thirty-eight patients with CRSwNP and ten healthy volunteers were included. The protein expression of multiple GR isoforms in nasal polyps (NPs) tissue and control mucosae was examined by western blot analysis with different GR antibodies. Results Five bands, including three bands for known proteins (GRα-A/B, GRα-C, and GRα-D) and two bands for unidentified proteins at 67 kilodaltons (kDa) and 60 kDa, were identified with both total GR antibody (PA1-511A) and GRα-specific antibody (PA1-516). GRα-D intensity, which was abundant in nasal mucosa, was significantly increased in the CRSwNP group and was especially elevated in the noneosinophilic CRSwNP (NE-CRSwNP) group (PA1-511A: P < 0.001 and P = 0.0018; PA1-516: P < 0.003 and P = 0.006, respectively). Additionally, the intensities of the newly recognized 67 kDa and 60 kDa bands were much greater in the NE-CRSwNP subgroup than in the eosinophilic CRSwNP (E-CRSwNP) subgroup; in the E-CRSwNP subgroup, the median intensities were even lower than those in the control group. Conclusions This study provides evidence that nasal tissues express multiple GR protein isoforms. GR protein isoforms presented disease and tissue-specific expression profiles that differed between the CRSwNP and control groups and between the E-CRSwNP and NE-CRSwNP subgroups. Graphical abstract

【 授权许可】

Unknown