Phytochemical Analysis and Biological Activities of the Ethanolic Extract of Daphne sericea Vahl Flowering Aerial Parts Collected in Central Italy

Biomolecules

Marisa Colone¹  Annarita Stringaro¹  Claudio Frezza²  Mauro Serafini²  Daniela De Vita²  Fabio Sciubba³  Marco Franceschin³  Alessandro Venditti³  Alessandra Gentili³  Pierpaolo Tomai³  Armandodoriano Bianco³  Antonella Di Sotto⁴  Giampiero Ciaschetti⁵  Mirella Di Cecco⁵ 

[1] Centro Nazionale per la Ricerca e la Valutazione Preclinica e Clinica dei Farmaci, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy;Dipartimento di Biologia Ambientale, Università di Roma “La Sapienza”, Piazzale Aldo Moro 5, 00185 Rome, Italy;Dipartimento di Chimica, Università di Roma “La Sapienza”, Piazzale Aldo Moro 5, 00185 Rome, Italy;Dipartimento di Fisiologia e Farmacologia “V. Erspamer”, Università di Roma “La Sapienza”, Piazzale Aldo Moro 5, 00185 Rome, Italy;Ufficio Monitoraggio e Conservazione della Biodiversità Vegetale, Parco Nazionale della Majella, Via Badia 28, 67039 Sulmona, Italy;

关键词: Daphne sericea Vahl;    Thymelaeaceae;    phytochemical analysis;    chemotaxonomy;    polyphenols;    antioxidant activities;   

DOI  :  10.3390/biom11030379

来源: DOAJ

【 摘 要 】

In this paper, the first phytochemical analysis of the ethanolic extract of Daphne sericea Vahl flowering aerial parts collected in Italy and its biological activities were reported. Eleven compounds were identified i.e., α-linolenic acid (1), tri-linoleoyl-sn-glycerol (2), pheophorbide a ethyl ester (3), pilloin (4), sinensetin (5), yuanhuanin (6), rutamontine (7), syringin (8), p-coumaric acid (9), p-anisic acid (10) and caffeic acid (11). To the best of our knowledge, compounds (1–4, 7–8 and 10) were isolated from D. sericea for the first time during this work, whereas sinensetin (5) represents a newly identified component of the entire Thymelaeaceae family. The extract was found to possess radical scavenging against both DPPH^• and 2,2’-azino-bis(3-thylbenzothiazoline-6-sulfonic acid (ABTS^•+) radicals, with at least a 40-fold higher potency against the latter. Moreover, chelating abilities against both ferrous and ferric ions have been highlighted, thus suggesting a possible indirect antioxidant power of the extract. Although the precise bioactive compounds remain to be discovered, the polyphenolic constituents, including phenolic acids, tannins and flavonoids, seem to contribute to the antioxidant power of the phytocomplex. In addition, the extract produced cytotoxic effects in MDA-MB-231 and U87-MG cancer cell lines, especially at the concentration of 625 μg/mL and after 48-72 h. Further studies are required to clarify the contribution of the identified compounds in the bioactivities of the extract and to support possible future applications.

【 授权许可】

Unknown