DnaK3 Is Involved in Biogenesis and/or Maintenance of Thylakoid Membrane Protein Complexes in the Cyanobacterium Synechocystis sp. PCC 6803

Life

Adrien Thurotte¹  Ruven Jilly¹  Dirk Schneider¹  Tobias Seidel¹  Uwe Kahmann² 

[1] Department of Chemistry, Biochemistry, Johannes Gutenberg University Mainz, 55128 Mainz, Germany;Department of Molecular Cell Biology, Bielefeld University, 33615 Bielefeld, Germany;

关键词: chaperone;    Hsp70;    photosynthesis;    thylakoid membrane biogenesis;    photosystem maintenance;    Synechocystis sp. PCC6803;   

DOI  :  10.3390/life10050055

来源: DOAJ

【 摘 要 】

DnaK3, a highly conserved cyanobacterial chaperone of the Hsp70 family, binds to cyanobacterial thylakoid membranes, and an involvement of DnaK3 in the biogenesis of thylakoid membranes has been suggested. As shown here, light triggers synthesis of DnaK3 in the cyanobacterium Synechocystis sp. PCC 6803, which links DnaK3 to the biogenesis of thylakoid membranes and to photosynthetic processes. In a DnaK3 depleted strain, the photosystem content is reduced and the photosystem II activity is impaired, whereas photosystem I is regular active. An impact of DnaK3 on the activity of other thylakoid membrane complexes involved in electron transfer is indicated. In conclusion, DnaK3 is a versatile chaperone required for biogenesis and/or maintenance of thylakoid membrane-localized protein complexes involved in electron transfer reactions. As mentioned above, Hsp70 proteins are involved in photoprotection and repair of PS II in chloroplasts.

【 授权许可】

Unknown