Novel membrane peptidase inhibitory peptides with activity against angiotensin converting enzyme and dipeptidyl peptidase IV identified from hen eggs

Journal of Functional Foods

Long Ding¹  Wenzhu Zhao²  Dan Zhang²  Zhipeng Yu²  Jingbo Liu³ 

[1] Corresponding author at: Jinzhou City, Liaoning Province, PR China.;College of Food Science and Engineering, Bohai University, Jinzhou 121013, PR China;College of Food Science and Engineering, Northwest A&F University, Yangling 712100, PR China;

关键词: Angiotensin converting enzyme inhibitory peptides;    Dipeptidyl peptidase IV inhibitory peptides;    Virtual screening;    Molecular docking;   

DOI  :  

来源: DOAJ

【 摘 要 】

Angiotensin converting enzyme (ACE) and dipeptidyl peptidase IV (DPP-IV) are important membrane peptidases. The inhibition of ACE and DPP-IV enzymes has become a key therapeutic target for the treatment of hypertension and diabetes. This study was conducted to discover membrane peptidase inhibitory peptides that inhibit ACE and DPP-IV. Egg proteins were cleaved by pepsin and trypsin in silico. The potential activity, solubility, absorption, distribution, metabolism, excretion, and toxicity of the peptides were then predicted online. Finally, ACE and DPP-IV were applied as molecular docking targets for the potential peptides. After simulating gastrointestinal digestion, the IC50 values of ADF, MIR, and FGR against ACE activity were 27.75 ± 0.90 mM, 24.97 ± 0.80 mM, and 66.98 ± 1.40 μM, against DPP-IV activity were 16.83 mM, 4.86 mM, and 46.22 mM, respectively. The ACE and DPP-IV inhibitory peptides identified from hen egg proteins can be used as functional food ingredients for controlling hypertension and diabetes.

【 授权许可】

Unknown