Modulation of Human Complement System by Antimicrobial Peptide Arenicin-1 from Arenicola marina

Marine Drugs

IliaA. Krenev¹  EkaterinaS. Umnyakova²  VladimirN. Kokryakov²  NikolayP. Gorbunov²  MikhailN. Berlov²  TatianaV. Ovchinnikova³  AlexanderV. Zhakhov⁴ 

[1] Department of Biochemistry, Saint-Petersburg State University, Universitetskaya Embankment, 7/9, Saint-Petersburg 199034, Russia;Institute of Experimental Medicine, Acad. Pavlov Str. 12, Saint Petersburg 197376, Russia;M.M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow 117997, Russia;Research Institute of Highly Pure Biopreparations, Pudozhskaya Str., 7, Saint Petersburg 197110, Russia;

关键词: Arenicola marina;    antimicrobial peptides;    arenicin;    complement;    C3a;   

DOI  :  10.3390/md16120480

来源: DOAJ

【 摘 要 】

Antimicrobial peptides from marine invertebrates are known not only to act like cytotoxic agents, but they also can display some additional activities in mammalian organisms. In particular, these peptides can modulate the complement system as was described for tachyplesin, a peptide from the horseshoe crab. In this work, we investigated the influence on complement activation of the antimicrobial peptide arenicin-1 from the marine polychaete Arenicola marina. To study effects of arenicin on complement activation in human blood serum, we used hemolytic assays of two types, with antibody sensitized sheep erythrocytes and rabbit erythrocytes. Complement activation was also assessed, by the level of C3a production that was measured by ELISA. We found that the effect of arenicin depends on its concentration. At relatively low concentrations the peptide stimulates complement activation and lysis of target erythrocytes, whereas at higher concentrations arenicin acts as a complement inhibitor. A hypothetical mechanism of peptide action is proposed, suggesting its interaction with two complement proteins, C1q and C3. The results lead to the possibility of the development of new approaches for therapy of diseases connected with complement dysregulation, using peptide regulators derived from natural antimicrobial peptides of invertebrates.

【 授权许可】

Unknown