In vitro inhibitory effect of chlorinated tetrasulfonated aluminum phthalocyanine against Leishmania (Viannia) Peruviana and Leishmania (Viannia) Braziliensis

Revista Peruana de Medicina Experimental y Salud Pública

卷:37

Alfredo Berrocal-Huallpa¹  Kelly Vanesa Izarra-Rojas²  Jorge León-Quispe³  José Luis Gonzáles-Medrano⁴  Julio Santiago-Contreras⁵  Nyshon Rojas-Palomino⁶  Gloria Minaya-Gómez⁷ 

[1] Facultad de Ciencias de la Salud, Universidad Autónoma de Ica, Perú.biólogo, magister en Mejoramiento Genéticode Plantas;

[2] Laboratorio de Ecología Microbiana, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Lima, Perú.Bióloga/o;

[3] Laboratorio de Ecología Microbiana, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Lima, Perú.biólogo, magíster en Ciencias Microbiológicas;

[4] Laboratorio de Investigación y Desarrollo de Química Orgánica, Universidad Nacional Mayor de San Marcos, Lima – Perú.químico;

[5] Laboratorio de Investigación y Desarrollo de Química Orgánica, Universidad Nacional Mayor de San Marcos, Lima, Perú.químico, doctor en Química;

[6] Laboratorio de Referencia Nacional de Leishmaniasis, Instituto Nacional de Salud, Lima, Perú.Bióloga/o;

[7] Laboratorio de Referencia Nacional de Leishmaniasis, Instituto Nacional de Salud, Lima, Perú.bióloga, máster en Medicina Tropical y Salud Internacional;

关键词: enfermedades tropicales;    leishmaniasis cutánea;    terapia fotodinámica;    fotosensibilizadores;    tratamiento alternativo;   

DOI  :  10.17843/rpmesp.2020.373.4465

来源: DOAJ

【 摘 要 】

Objectives: To evaluate the in vitro photodynamic activity of aluminum phthalocyanine tetrasulfonate chloride (AlPc- ClS4) on promastigotes and amastigotes of Leishmania (Viannia) peruviana and Leishmania (Viannia) braziliensis. Materials and methods: The activity of photodynamic therapy using AlPcClS4 on Leishmania promastigote and amastigotes was determined by the Methyl Thiazole Tetrazolium (MTT) colorimetric method and quantitative PCR, respectively. Results: Photodynamic treatment showed an inhibitory effect on promastigotes, particularly on Leishmania (V.) peruviana, to a lesser extent on Leishmania (V.) braziliensis and also on intracellular forms of both species. At 24 hours post-radiation, using concentrations of 200 μM and 350 μM, the inhibitory effect on Leishmania (V.) peruviana was 72.9% and 73.9% respectively; at 96 hours the inhibitory effect was of 78.8% and 80.6%, respectively. Regarding intracellular forms, the inhibitory effect on Leishmania (V.) peruviana amastigotes was 57.8% at 72 hours post-treatment, using a concentration of 200 μM. The IC50 was 56.5, 50, 44 and 39.7 μM, at 24, 48, 72 and 96 hours post-radiation, respectively. Conclusions: Photodynamic therapy using AlPcClS4 against Leishmania species showed encouraging results, mainly on Leishmania (V.) peruviana, suggesting a potential use as an alternative or complement to the usual treatment of tegumentary leishmaniasis. However, new trials are still required to determine the selectivity index for the intracellular form of the parasite, and to develop methods to facilitate the efficient entry of the molecule into the host cell and the parasite.

【 授权许可】

Unknown