Acta Dermato-Venereologica | 卷:100 |
Autoantibody Profile of a Cohort of 54 Italian Patients with Linear IgA Bullous Dermatosis: LAD-1 Denoted as a Major Auto-antigen of the Lamina Lucida Subtype | |
Emanuele Cozzani1  Giulia Gasparini2  Adele Salemme2  Clara De Simone2  Arianna Fay Agnoletti2  Angelo V. Marzano2  Marzia Caproni2  Emiliano Antiga2  Aurora Parodi2  Giovanni di Zenzo2  Corrado Ocella2  Riccardo Cavalli2  Camilla Vassallo2  | |
[1] Section of Dermatology, Department of Health Sciences (Di.S.Sal.), University of Genoa, 16132 Genoa, Italy. emanuele.cozzani@unige.it. ; | |
[2] ; | |
关键词: linear iga bullous disease; humoral immune response; lad-1; bp180; bp230; diagnostic sensitivity; | |
DOI : 10.2340/00015555-3415 | |
来源: DOAJ |
【 摘 要 】
Linear IgA bullous dermatosis (LABD) is characterized by presence of multiple IgA autoantibodies, and a comparatively lesser number of IgG antibodies, directed against different hemidesmosomal antigens. The main autoantigens are LAD-1, LABD-97, BP180 and BP230, type VII collagen and laminin 332. We retrospectively studied the serology of 54 Italian patients with LABD using enzyme-linked immunosorbent assay (ELISA), immunoblotting assay, and indirect immunofluorescence on monkey oesophagus and salt-split skin. Among these, indirect immunofluorescence of salt-split skin elicits the greatest sensitivity. Sixty-three percent of the sera were observed to be positive, with a lamina lucida pattern observed in 48%, a sub-lamina densa pattern in 2% and a mixed pattern in 13% of the cases. IgA reactivity to LAD-1 on immunoblotting was found in 52% of sera, to BP180-NC16A by ELISA in 32% and to BP230 in 26%. Only 17% of patients possessed circulating IgG autoantibodies. LAD-1 was determined to be a major autoantigen of the lamina lucida subtype. Combined serological assays demonstrated a high sensitivity (82%), suggesting that this approach could support diagnosis when a biopsy is not feasible or direct immunofluorescence results are negative.
【 授权许可】
Unknown