Cytoplasmic and Nuclear TAZ Exert Distinct Functions in Regulating Primed Pluripotency

Stem Cell Reports

卷:9

Jean Paul Chadarevian¹  Xingliang Zhou¹  Qi-Long Ying¹  Bryan Ruiz¹ 

[1] Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA;

关键词: β-catenin;    TAZ;    Hippo pathway;    Wnt signaling pathway;    epiblast stem cell;    human embryonic stem cell;    primed pluripotency;    stem cell self-renewal;   

DOI  :  10.1016/j.stemcr.2017.07.019

来源: DOAJ

【 摘 要 】

Mouse epiblast stem cells (mEpiSCs) and human embryonic stem cells (hESCs) are primed pluripotent stem cells whose self-renewal can be maintained through cytoplasmic stabilization and retention of β-catenin. The underlying mechanism, however, remains largely unknown. Here, we show that cytoplasmic β-catenin interacts with and retains TAZ, a Hippo pathway effector, in the cytoplasm. Cytoplasmic retention of TAZ promotes mEpiSC self-renewal in the absence of nuclear β-catenin, whereas nuclear translocation of TAZ induces mEpiSC differentiation. TAZ is dispensable for naive mouse embryonic stem cell (mESC) self-renewal but required for the proper conversion of mESCs to mEpiSCs. The self-renewal of hESCs, like that of mEpiSCs, can also be maintained through the cytoplasmic retention of β-catenin and TAZ. Our study indicates that how TAZ regulates cell fate depends on not only the cell type but also its subcellular localization.

【 授权许可】

Unknown