Simultaneous Application of Biosurfactant and Bioaugmentation with Rhamnolipid-Producing Shewanella for Enhanced Bioremediation of Oil-Polluted Soil

Applied Sciences

卷:9

Abitha Benson¹  Tongmin Sa²  Parthasarathi Rengasamy³  Ram Gomathi⁴  Manoharan Melvin Joe⁴  Devaraj Shalini⁴  Allen John Henry⁴  Marika Truu⁵  Jaak Truu⁵ 

[1] Department of Biotechnology, School of Life Sciences, VELS University, Velan Nagar, Pallavaram, Chennai 600117, Tamilnadu, India;

[2] Department of Environmental and Biological Chemistry, Chungbuk National University, Cheongju 28644, Korea;

[3] Department of Microbiology, Faculty of Agriculture, Annamalainagar 608002, Tamilnadu, India;

[4] Department of Microbiology, School of Life Sciences, VELS University, Velan Nagar, Pallavaram, Chennai 600117, Tamilnadu, India;

[5] Institute of Molecular and Cell Biology, University of Tartu, Riia 23, EE51010 Tartu, Estonia;

关键词: bioremediation;    biosurfactant;    hydrocarbon-contaminated soil;    rhamnolipids;    Shewanella sp.;   

DOI  :  10.3390/app9183773

来源: DOAJ

【 摘 要 】

In the present study, a combined treatment strategy involving the addition of rhamnolipid, rhamnolipid-producing bacteria (Shewanella sp. BS4) and a native soil microbial community for the remediation of hydrocarbon-contaminated soil under pilot-scale conditions was adopted. The isolate BS4 (rhl+), demonstrating the highest emulsification activity and surface tension reduction efficiency, was identified based on 16 S rDNA sequencing as Shewanella sp. strain. Growth conditions for rhamnolipid production were optimized based on Central Composite Design (CCD) as 2.9% crude oil, a 54 × 10⁶ CFU g⁻¹ inoculation load of soil, a temperature of 30.5 °C, and a pH of 6.7. In situ bioremediation experiments, conducted using hydrocarbon-contaminated soil treated with the combination of rhamnolipid and rhamnolipid-producing bacteria, showed that the inoculated Shewanella sp. BS4, along with the indigenous soil microbial community, supported the highest hydrocarbon-degrading bacterial population and soil respiration activity, and this treatment resulted in 75.8% hydrocarbon removal efficiency, which was higher compared to contaminated soil devoid of any treatment.

【 授权许可】

Unknown