Factors Influencing Sulforaphane Content in Broccoli Sprouts and Subsequent Sulforaphane Extraction

Foods

卷:10

Martin Magner¹  Petr Šnurkovič²  Pavel Híc²  Josef Balík²  Ivo Soural²  Pavla Novotná³  Milan Houška³  Jan Tříska⁴  Naděžda Vrchotová⁴  Kateřina Thorová⁵  Ladislav Jílek⁶ 

[1] Department of Pediatrics, University Thomayer Hospital, First Faculty of Medicine, Charles University, 140 59 Prague 4, Czech Republic;

[2] Faculty of Horticulture, Mendel University in Brno, 691 44 Lednice, Czech Republic;

[3] Food Research Institute Prague (FRIP), 102 00 Prague 10, Czech Republic;

[4] Global Change Research Institute CAS, 603 00 Brno, Czech Republic;

[5] National Institute for Autism, 182 00 Prague 8, Czech Republic;

[6] Pure Food Norway, 1400 Ski, Norway;

关键词: broccoli and radish sprouts;    sulforaphane;    myrosinase;    extraction;    epithiospecifier protein;   

DOI  :  10.3390/foods10081927

来源: DOAJ

【 摘 要 】

Broccoli sprouts contain 10–100 times higher levels of sulforaphane than mature plants, something that has been well known since 1997. Sulforaphane has a whole range of unique biological properties, and it is especially an inducer of phase 2 detoxication enzymes. Therefore, its use has been intensively studied in the field of health and nutrition. The formation of sulforaphane is controlled by the epithiospecifier protein, a myrosinase co-factor, which is temperature-specific. This paper studies the influence of temperature, heating time, the addition of myrosinase in the form of Raphanus sativus sprouts in constant ratio to broccoli sprouts, and other technological steps on the final sulforaphane content in broccoli sprout homogenates. These technological steps are very important for preserving sulforaphane in broccoli sprouts, but there are some limitations concerning the amount of sulforaphane. We focused, therefore, on the extraction process, using suitable β-cyclodextrin, hexane and ethanol, with the goal of increasing the amount of sulforaphane in the final extract, thus stabilizing it and reducing the required amount sulforaphane needed, e.g., as a dietary supplement.

【 授权许可】

Unknown