Sclerotium Rot of Pulsatilla koreana Nakai Caused by Sclerotium rolfsii

Research in Plant Disease

卷:21

Jin-Hyeuk Kwon¹  Sang-Dae Lee¹  Gap-Chun Hwang¹  Yong-Jo Choi¹  Seol-Bi Lee¹  Le-Minh Nguyen² 

[1] 1Gyeongsangnam-do Agricultural Research and Extension Services, Jinju 660-360, Korea,;

[2] 2Plant Protection Subdepartment of Dongnai, Dongnai Province 710000, Vietnam;

关键词: Pulsatilla koreana;    Sclerotium rolfsii;   

DOI  :  http://dx.doi.org/10.5423/RPD.2015.21.4.334

来源: DOAJ

【 摘 要 】

Sclerotium rot on Pulsatilla koreana was observed in the exhibition field of Jinju Agriculture Technology Center in July 2013. The infected plants showed water-soaked, blighted and rotted symptoms. White mycelial mats spread over lesions, and then numerous sclerotia were formed on flower stalk and flower stem near the soil line. The sclerotia were globoid in shape, 1-3 mm in size and white to brown in color. The optimum temperature for mycelial growth and sclerotia formation on PDA was 30°C and the hyphal width was 4-8 μm. The typical clamp connections were observed in the hyphae of the fungus grown on PDA. For molecular identification, the complete ITS rDNA sequence of the causal fungus was sequenced and analyzed. On the basis of mycological characteristics, ITS rDNA sequence analysis, and pathogenicity to host plants, this fungus was identified as Sclerotium rolfsii Saccardo. This is the first report of sclerotium rot on Pulsatilla koreana caused by S. rolfsii in Korea.

【 授权许可】

Unknown