Leafy Stems of Phagnalon saxatile subsp. saxatile from Algeriaas a Source of Chlorogenic Acids and Flavonoids with Antioxidant Activity: Characterization and Quantification Using UPLC-DAD-ESI-MSn

Metabolites

卷:11

Romain Larbat¹  Riadh Ksouri²  TarikMohammed Chaouche³  Farah Haddouchi³ 

[1] Laboratoire Agronomie et Environnement, Université de Lorraine, INRAE, F-54000 Nancy, France;

[2] Laboratory of Aromatic and Medicinal Plants, Biotechnology Center of Borj-Cédria, B.P 901, Hammam-Lif 2050, Tunisia;

[3] Natural Products Laboratory, Department of Biology, Abou Bekr Belkaid University, B.P 119, Tlemcen 13000, Algeria;

关键词: Phagnalon saxatile subsp. saxatile;    phenolic compounds;    polarity;    antioxidant activities;    UPLC-DAD-ESI-MSⁿ;    chlorogenic acids;   

DOI  :  10.3390/metabo11050280

来源: DOAJ

【 摘 要 】

Phagnalon saxatile subsp. saxatile is a wild species widespread in Algeria which is utilized for medicinal purposes as analgesic and anticholesterolemic. However, information is still scarce regarding its phytochemical content. The objective of this study was to identify and quantify the phenolic compounds from different extracts of its leafy stems. For this purpose, the effects of four extracting solvents were investigated on the content of phenolic compounds and the antioxidant activity of this plant. The extracts prepared with polar solvents (methanol and water) contained higher amounts of phenolic compounds and showed better antioxidant activity than the extracts with apolar solvents (hexane, dichloromethane). The methanolic extract, richest in total phenolic and total flavonoid, had significant antioxidant activity as regarded by DPPH° scavenging capacity (IC₅₀ of 5.5 µg/mL), ABTS⁺° scavenging capacity (IC₅₀ of 63.8 µg/mL) and inhibition of oxidation of linoleic acid (IC₅₀ of 22.7 µg/mL), when compared to synthetic antioxidants. Chlorogenic acids and several flavonoids were identified and quantified by UPLC-DAD-MSⁿ. The di-O-caffeoylquinic acids isomers were the most concentrated phenolics (25.4 mg/g DW) in the methanolic extract.

【 授权许可】

Unknown