期刊论文详细信息
International Journal of Molecular Sciences 卷:21
3-D Nucleus Architecture in Oat × Maize Addition Lines
Edyta Skrzypek1  Kinga Dziurka1  Marzena Warchoł1  Ilona Czyczyło-Mysza1  Tomasz Warzecha2  Dominika Idziak-Helmcke3  Marta Sowa3 
[1] Department of Biotechnology, The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Kraków, Poland;
[2] Department of Plant Breeding, Physiology, and Seed Science, University of Agriculture in Kraków, Podłużna 3, 30-239 Kraków, Poland;
[3] Institute of Biology, Biotechnology, and Environmental Sciences, University of Silesia in Katowice, Jagiellońska 28, 40-032 Katowice, Poland;
关键词: addition line;    Avena sativa L.;    chromosome territory;    GISH;    hybrid;    nucleus;   
DOI  :  10.3390/ijms21124280
来源: DOAJ
【 摘 要 】

The nucleus architecture of hybrid crop plants is not a well-researched topic, yet it can have important implications for their genetic stability and usefulness in the successful expression of agronomically desired traits. In this work we studied the spatial distribution of introgressed maize chromatin in oat × maize addition lines with the number of added maize chromosomes varying from one to four. The number of chromosome additions was confirmed by genomic in situ hybridization (GISH). Maize chromosome-specific simple sequence repeat (SSR) markers were used to identify the added chromosomes. GISH on 3-D root and leaf nuclei was performed to assess the number, volume, and position of the maize-chromatin occupied regions. We revealed that the maize chromosome territory (CT) associations of varying degree prevailed in the double disomic lines, while CT separation was the most common distribution pattern in the double monosomic line. In all analyzed lines, the regions occupied by maize CTs were located preferentially at the nuclear periphery. A comparison between the tissues showed that the maize CTs in the leaf nuclei are positioned closer to the center of the nucleus than in the root nuclei. These findings shed more light on the processes that shape the nucleus architecture in hybrids.

【 授权许可】

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