From Food Waste to Innovative Biomaterial: Sea Urchin-Derived Collagen for Applications in Skin Regenerative Medicine

Marine Drugs

卷:18

Moira Paroni¹  Paolo Landini¹  Raffaella Macchi¹  Graziano Colombo¹  Chiara Gomiero²  Luca Melotti²  Marco Patruno²  Cinzia Ferrario³  Francesco Bonasoro³  Francesco Rusconi³  Michela Sugni³  Albana Pulaj³  M.Daniela Candia Carnevali³  Tiziana Martinello⁴ 

[1] Department of Biosciences, University of Milan, Via Celoria, 26, 20133 Milan, Italy;

[2] Department of Comparative Biomedicine and Food Science, University of Padua, Agripolis Viale dell’Università, 16, 35020 Legnaro, Italy;

[3] Department of Environmental Science and Policy, University of Milan, Via Celoria, 2, 20133 Milan, Italy;

[4] Department of Veterinary Medicine, University of Bari, SP. Casamassima Km.3, 70010 Valenzano, Bari, Italy;

关键词: fibrillar collagen;    sea urchins;    marine collagen-based skin-like scaffolds;    eco-friendly biomaterial;    regenerative medicine;   

DOI  :  10.3390/md18080414

来源: DOAJ

【 摘 要 】

Collagen-based skin-like scaffolds (CBSS) are promising alternatives to skin grafts to repair wounds and injuries. In this work, we propose that the common marine invertebrate sea urchin represents a promising and eco-friendly source of native collagen to develop innovative CBSS for skin injury treatment. Sea urchin food waste after gonad removal was here used to extract fibrillar glycosaminoglycan (GAG)-rich collagen to produce bilayer (2D + 3D) CBSS. Microstructure, mechanical stability, permeability to water and proteins, ability to exclude bacteria and act as scaffolding for fibroblasts were evaluated. Our data show that the thin and dense 2D collagen membrane strongly reduces water evaporation (less than 5% of water passes through the membrane after 7 days) and protein diffusion (less than 2% of BSA passes after 7 days), and acts as a barrier against bacterial infiltration (more than 99% of the different tested bacterial species is retained by the 2D collagen membrane up to 48 h), thus functionally mimicking the epidermal layer. The thick sponge-like 3D collagen scaffold, structurally and functionally resembling the dermal layer, is mechanically stable in wet conditions, biocompatible in vitro (seeded fibroblasts are viable and proliferate), and efficiently acts as a scaffold for fibroblast infiltration. Thus, thanks to their chemical and biological properties, CBSS derived from sea urchins might represent a promising, eco-friendly, and economically sustainable biomaterial for tissue regenerative medicine.

【 授权许可】

Unknown