A Novel Disulfide-Containing Polycationic Amphiphile: 1,28-Di[(cholest-5-en-3β-yl)disulfanyl]-4,25-dioxo-3,8,12,17,21,26-hexaazaoctacosane Tetrahydrochloride

Molbank

卷:2018

Marina A. Zenkova¹  Valeria D. Andreeva²  Nina G. Morozova²  Mikhail A. Maslov²  Pavel A. Puchkov²  Elena V. Shmendel² 

[1] Institute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentiev ave. 8, 630090 Novosibirsk, Russia;

[2] Lomonosov Institute of Fine Chemical Technologies, Moscow Technological University, Vernadsky ave. 86, 119571 Moscow, Russia;

关键词: redox-sensitive;    disulfide linker;    gemini amphiphiles;    cationic liposomes;    gene therapy;   

DOI  :  10.3390/M981

来源: DOAJ

【 摘 要 】

The absence of highly effective delivery systems is a major challenge for gene therapy. Our work was aimed at the development of novel cationic liposomes possessing high transfection efficiency. For this purpose, a novel disulfide polycationic amphiphile 2S4 was synthesized. Cationic liposomes based on 2S4 and a helper lipid DOPE were formed by the thin film hydration method and exhibited effective plasmid DNA delivery into the HEK293 cells, with a transfection activity superior to that of the commercial agent Lipofectamine® 2000. Our results suggest that the polycationic amphiphile 2S4 is a promising candidate for in vitro nucleic acid delivery.

【 授权许可】

Unknown