Potential urine proteomics biomarkers for primary nephrotic syndrome

Clinical Proteomics

卷:14

Min-Young Song¹  Kang-Sik Park¹  Ju-Young Moon²  Chun-Gyoo Ihm²  Sang-Ho Lee²  Tae-Won Lee²  Kyung-Hwan Jeong²  Young Wook Choi²  Yang Gyun Kim² 

[1] Department of Physiology, Kyung Hee University School of Medicine;

[2] Division of Nephrology, Department of Internal Medicine, Kyung Hee University School of Medicine;

关键词: Focal and segmental glomerulosclerosis;    Minimal change disease;    Membranous nephropathy;    Nephrotic syndrome;    Urine proteomics;   

DOI  :  10.1186/s12014-017-9153-1

来源: DOAJ

【 摘 要 】

Abstract Background Nephrotic syndrome (NS) is a nonspecific kidney disorder, commonly caused by minimal change disease (MCD), focal segmental glomerulosclerosis (FSGS), and membranous nephropathy (MN). Here we analyzed urinary protein profiles, aiming to discover disease-specific biomarkers of these three common diseases in NS. Methods Sixteen urine samples were collected from patients with biopsy-proven NS and healthy controls. After removal of high-abundance proteins, the urinary protein profile was analyzed by LC–MS/MS to generate a discovery set. For validation, ELISA was used to analyze the selected proteins in 61 urine samples. Results The discovery set included 228 urine proteins, of which 22 proteins were differently expressed in MCD, MN, and FSGS. Among these, C9, CD14, and SERPINA1 were validated by ELISA. All three proteins were elevated in MCD, MN, and FSGS groups compared with in IgA nephropathy and healthy controls. When a regression model was applied, receiver operating characteristic analysis clearly discriminated MCD from the other causative diseases in NS. Conclusions We developed a disease-specific protein panel that discriminated between three main causes of NS. Through this pilot study, we suggest that urine proteomics could be a non-invasive and clinically available tool to discriminate MCD from MN and FSGS.

【 授权许可】

Unknown