Placental Abnormalities are Associated With Specific Windows of Embryo Culture in a Mouse Model

Frontiers in Cell and Developmental Biology

卷:10

Richard M. Schultz¹  Kashviya V. Suri²  Eric A. Rhon-Calderon²  Asha K. Dahiya²  Marisa S. Bartolomei²  Yemin Lan²  Lisa A. Vrooman³ 

[1] Department of Biology, School of Arts and Sciences, University of Pennsylvania, Philadelphia, PA, United States;

[2] Department of Cell and Developmental Biology, Perelman School of Medicine, Epigenetics Institute, University of Pennsylvania, Philadelphia, PA, United States;

[3] Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, OR, United States;

关键词: embryo culture;    assisted reproductive technologies (ART);    preimplantation embryo;    placenta;    imprinted gene;    perinatal outcome;   

DOI  :  10.3389/fcell.2022.884088

来源: DOAJ

【 摘 要 】

Assisted Reproductive Technologies (ART) employ gamete/embryo handling and culture in vitro to produce offspring. ART pregnancies have an increased risk of low birth weight, abnormal placentation, pregnancy complications, and imprinting disorders. Embryo culture induces low birth weight, abnormal placental morphology, and lower levels of DNA methylation in placentas in a mouse model of ART. Whether preimplantation embryos at specific stages of development are more susceptible to these perturbations remains unresolved. Accordingly, we performed embryo culture for several discrete periods of preimplantation development and following embryo transfer, assessed fetal and placental outcomes at term. We observed a reduction in fetal:placental ratio associated with two distinct windows of preimplantation embryo development, one prior to the morula stage and the other from the morula to blastocyst stage, whereas placental morphological abnormalities and reduced imprinting control region methylation were only associated with culture prior to the morula stage. Extended culture to the blastocyst stage also induces additional placental DNA methylation changes compared to embryos transferred at the morula stage, and female concepti exhibited a higher loss of DNA methylation than males. By identifying specific developmental windows of susceptibility, this study provides a framework to optimize further culture conditions to minimize risks associated with ART pregnancies.

【 授权许可】

Unknown