Dielectrophoretic characterization of dendritic cell deformability upon maturation

BioTechniques

卷:70

Mohammad A Qasaimeh¹  Abhishek Vembadi²  Aseel Alatoom³  Jiranuwat Sapudom⁴  Anoop Menachery⁵  Jeremy Teo⁶ 

[1] Applications, Division of Engineering, New York University Abu Dhabi, Abu Dhabi, UAE;

[2] Electronics, Malta College for Arts, Science &

[3] Technology, Malta;

[4] Transport, Electrical &

[5] 1Institute of Engineering &

[6] 2Laboratory for Immuno Bioengineering Research &

关键词: actin;    biochip;    cytoskeleton;    deformability;    dendritic cells;    dielectrophoresis;   

DOI  :  10.2144/btn-2020-0126

来源: DOAJ

【 摘 要 】

We have developed a rapid technique for characterizing the biomechanical properties of dendritic cells using dielectrophoretic forces. It is widely recognized that maturing of dendritic cells modulates their stiffness and migration capabilities, which results in T-cell activation triggering the adaptive immune response. Therefore it is important to develop techniques for mechanophenotyping of immature and mature dendritic cells. The technique reported here utilizes nonuniform electric fields to exert a substantial force on the cells to induce cellular elongation for optical measurements. In addition, a large array of interdigitated electrodes allows multiple cells to be stretched simultaneously. Our results indicate a direct correlation between F-actin activity and deformability observed in dendritic cells, determined through mean fluorescence signal intensity of phalloidin.

【 授权许可】

Unknown